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Choosing a diluent, working out the concentration, and the volume to add for each compound we supply.
Peptides ship as a dry powder because that is the form that survives storage and shipping.
A lyophilised peptide has had its water removed under vacuum, which leaves a stable cake or film at the bottom of the vial. In that state the molecule is largely inert: the chemistry that degrades a peptide mostly needs water to proceed. Reconstitution is simply putting that water back, in a measured amount, immediately before the compound is used.
The amount you add is the only variable you control, and it decides everything downstream. The mass of peptide in the vial is fixed by what you bought. Add less water and each drop carries more compound; add more and it carries less. Nothing is gained or lost - you are choosing the scale you will be measuring on for the rest of that vial's life.
Because of that, it is worth deciding the volume deliberately rather than defaulting to whatever is convenient. A volume that puts your intended dose on a round number is far easier to work with than one that lands you between graduations every time.
For anything you will draw from more than once, bacteriostatic water is the sensible default.
Bacteriostatic water is sterile water carrying roughly 0.9% benzyl alcohol. The alcohol does not sterilise anything, but it does suppress microbial growth, which matters the moment a stopper has been pierced. Every withdrawal is another opportunity for contamination, so a multi-use container wants that protection.
Sterile water contains no preservative at all. It is perfectly clean in a sealed ampoule and entirely unprotected once opened, which makes it a single-use proposition. It is the right choice only when the whole vial is being consumed at once.
Bacteriostatic saline turns up occasionally. The added sodium chloride is not inert as far as the peptide is concerned, and some sequences are sensitive to the change in ionic strength, so it is not a drop-in substitute.
Whichever you use, the diluent should be clear and in date. If a vial of water looks cloudy or has anything visible floating in it, that question is settled before the peptide is involved.
Two divisions cover almost everything you will need to work out.
Concentration is the peptide mass divided by the water you added. Reconstitute a 20 mg vial with 2 mL and you have 10 mg/mL. Use 4 mL instead and you have 5 mg/mL, from the same vial.
peptide mass ÷ water added = concentration
target dose ÷ concentration = volume to draw
The second line runs the first one backwards. At 10 mg/mL, a 0.5 mg dose is 0.05 mL. On a U-100 insulin syringe, where 100 units is 1 mL, that is 5 units - the unit scale is just hundredths of a millilitre with a friendlier name.
A prefilled pen works on the same arithmetic but removes the measuring step. Each click of the dial dispenses 0.01 mL, so once the concentration is set, one click is always the same mass and you are counting clicks rather than reading a barrel. The dose calculator does this conversion for any compound we carry.
The powder is more fragile than it looks, and most damage happens in the thirty seconds after the water goes in.
Let a refrigerated vial come up to room temperature before you open anything. A cold surface pulls moisture out of the air, and you do not want that condensing inside a vial you are about to open.
Swab both stoppers with alcohol and let them dry. Draw your measured diluent, then angle the needle so the water runs down the inside wall of the vial rather than landing directly on the powder. A jet fired straight into the cake drives it into suspension violently, and that is where aggregation starts.
Then leave it alone for a moment. Most peptides go into solution on their own within a minute or two. If it needs help, roll the vial gently between your palms or swirl it - never shake it. Shaking whips air through the liquid, and the air-liquid interface is where peptide chains unfold, stick to each other, and drop out of solution as aggregates you cannot redissolve.
When it is clear, label the vial with the compound, the concentration you just created, and the date. Concentration is the one thing that cannot be recovered by looking at the vial later, and a week from now you will not remember it.
A Klik-pen doses by fixed increments, so the water you add sets the size of every click.
With a syringe you can compensate for an odd concentration by drawing an odd volume. A pen cannot: it advances in discrete steps of 0.01 mL, and there is nothing between one click and the next. That makes the reconstitution volume a genuine decision rather than a preference.
Reconstitute a 20 mg pen with 2 mL and each click delivers 0.1 mg - a 0.4 mg dose is four clicks exactly. Use 3 mL and each click is 0.067 mg, so the same dose is six clicks and a bit, and you are rounding on every single dose for the life of the pen.
This is why the volumes in the table below are worth following. They are the figures each product's own click guide is built on, so using them means the numbers printed on the product page are the numbers you will actually get.
Generated from the live catalogue, so it stays in step with what is actually on the shelf.
| Compound | Formats | Contains | Add water | Strength | Per click |
|---|---|---|---|---|---|
| 51MQ | Vial · Pen | 100 mg | 3.0 mL | 33.33 mg/mL | 333 mcg |
| ALPHA | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| AOD | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| BPC-157 | Pen · Vial | 25 mg | 3.0 mL | 8.33 mg/mL | 83 mcg |
| CAR | Pen · Vial | 50 mg | 3.0 mL | 16.67 mg/mL | 167 mcg |
| CJC | Pen · Vial | 20 mg | 2.0 mL | 10.00 mg/mL | 100 mcg |
| EPILON | Pen · Vial | 5 mg | 2.0 mL | 2.50 mg/mL | 25 mcg |
| FRAG | Pen · Vial | 5 mg | 2.0 mL | 2.50 mg/mL | 25 mcg |
| GHK | Pen · Vial | 100 mg | 3.0 mL | 33.33 mg/mL | 333 mcg |
| GHKCU-Nasal | Nasal | 100 mg | 3.0 mL | 33.33 mg/mL | 333 mcg |
| GLOW | Pen · Vial | 80 mg | 3.0 mL | 26.67 mg/mL | 267 mcg |
| GLP-3 Reta | Vial · Pen | 30 mg | 2.4 mL | 12.50 mg/mL | 125 mcg |
| GLP-4 Reta | Pen · Vial | 35 mg | 2.4 mL | 14.58 mg/mL | 146 mcg |
| GLUTA | Vial · Pen | 2,000 mg | 3.0 mL | 666.67 mg/mL | 6.67 mg |
| IPAM | Pen · Vial | 20 mg | 2.0 mL | 10.00 mg/mL | 100 mcg |
| JARO | Pen · Vial | 60 mg | 3.0 mL | 20.00 mg/mL | 200 mcg |
| KLOW | Pen · Vial | 85 mg | 3.0 mL | 28.33 mg/mL | 283 mcg |
| KPV | Pen · Vial | 30 mg | 3.0 mL | 10.00 mg/mL | 100 mcg |
| MOTS-C | Vial · Pen | 40 mg | 3.0 mL | 13.33 mg/mL | 133 mcg |
| MT2 | Pen · Vial | 15 mg | 2.0 mL | 7.50 mg/mL | 75 mcg |
| NAD+ | Pen · Vial | 500 mg | 3.0 mL | 166.67 mg/mL | 1.67 mg |
| PAM1295 | Pen · Vial | 40 mg | 3.0 mL | 13.33 mg/mL | 133 mcg |
| PEPTIN | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| PT141 | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| PT141-NASAL | Nasal | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| RETA 60 | Pen · Vial | 60 mg | 2.4 mL | 25.00 mg/mL | 250 mcg |
| RP2 | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| RP6 | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| SELANK | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| SELANK-NASAL | Nasal | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| SEMAX | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| SEMAX-NASAL | Nasal | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| SERM | Pen · Vial | 5 mg | 2.0 mL | 2.50 mg/mL | 25 mcg |
| SLUP332 | Pen · Vial | 20 mg | 2.0 mL | 10.00 mg/mL | 100 mcg |
| SS31 | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| TB-500 | Vial · Pen | 25 mg | 3.0 mL | 8.33 mg/mL | 83 mcg |
| TB157 | Pen · Vial | 50 mg | 3.0 mL | 16.67 mg/mL | 167 mcg |
| TESA | Pen · Vial | 20 mg | 2.0 mL | 10.00 mg/mL | 100 mcg |
| TIDE | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| VIP | Pen · Vial | 10 mg | 2.0 mL | 5.00 mg/mL | 50 mcg |
| ZEMPIC | Pen | 2 mg | 2.0 mL | 1.00 mg/mL | 10 mcg |
Volumes marked here follow the reconstitution figures published on each product page. Where a compound has no published figure we suggest a volume that keeps a single click to a workable fraction of a dose - you can use a different volume, and the calculator will follow it.
A reconstituted peptide is on a much shorter clock than the powder was.
Dry powder is stable for months to years depending on how cold you keep it. In solution, that window shrinks to weeks. Refrigerate at 2-8 °C from the moment it is mixed, keep it out of the light, and treat roughly 28 days as a conservative working life - longer is often fine if the vial has stayed sterile and the solution has stayed clear, but that is a judgement rather than a guarantee.
Inspect it before each use. Cloudiness, visible particles, a gel-like texture, or anything clinging to the glass all mean the same thing: discard it. There is no procedure for rescuing a peptide that has aggregated, and a solution that looks wrong is not worth the uncertainty.
If you need a vial to last past that window, the answer is to aliquot into single-use portions and freeze them at the point of reconstitution, not to stretch one vial. Full detail on temperatures and freeze-thaw is in the storage guide.
This page is general laboratory reference material for research use. It is not medical advice and not a dosing protocol. Figures are drawn from our own product documentation; confirm against the vial in front of you before you rely on them.